KASP™ chemistry can be used for the detection of the following polymorphism types: SNPs or indels, presence or absence of target DNA sequence (plus/minus assay).
Learn moreOnce your project manager has received your SNP submission template, KASP™ genotyping assays will be designed using our Kraken™ software.
Learn moreLGC, Biosearch Technologies requires to know the layouts of your DNA sample plates; please submit DNA sample plate data using the appropriate DNA submission template.
Learn morePlease follow our guidelines to ensure that you send sufficient DNA for your genotyping project, and that it arrives at LGC, Biosearch Technologies in good condition.
Learn moreOnce your KASP™ assays have been prepared and your DNA samples are received, laboratory work will commence for your genotyping project.
Learn moreExpress turnaround times for genotyping and DNA extraction service projects.
Learn moreOnce your genotyping project is complete, you will receive results. The results file will be in an Excel .csv (comma-separated text file) format.
Learn moreThe customer submits a request for a quotation or a general request for pricing information. When requesting this, please include details regarding the number of SNPs (or indels) and number of DNA samples that you wish to genotype. LGC Biosearch Technologies will email the customer with a quotation for their genotyping project and will include a sequence submission form and a DNA sample submission form.
The customer reviews their quotation and if happy, submits a purchase order to the provided address.
At this stage Biosearch Technologies will assign a project manager to act as the customers’ primary contact for this and any subsequent genotyping projects.
Next, the customer should complete and return the sequence submission form with sequence(s) of interest and polymorphism(s) marked to identify the SNPs/indels that they are interested in. The completed sequence submission form can be emailed directly to the project manager.
Biosearch Technologies uses the sequence information submitted by the customer to design the KASP assays; for this, we use our proprietary Kraken software. The oligos are ordered, and KASP assays are prepared. These assays are validated against our panel of DNA samples where applicable (human assays only.
Meanwhile, the customer will send their DNA samples to Biosearch Technologies, following our guidelines on sample shipment. Customers must ensure that they have completed a DNA sample submission form to identify their samples and emailed it to the project manager.
Once DNA has been received, LGC prepare sample plates for genotyping using either 384-well or 1536-well format plates depending on the number of SNPs/indels and samples being genotyped.
When all of the sample plates have been prepared and the assays have been made (and validated) the assays will be dispensed into the genotyping sample plates. The plates are then sealed, and thermally cycled in one of our Hydrocycler instruments. Plates are then read to obtain fluorescence data.
The fluorescence data read from the sample plates is plotted and genotypes are assigned to samples based on clusters.
Finally, the project manager will send the analysed results to the customer. Results are typically provided in two formats, one that can be viewed graphically using our SNPViewer software and the other that displays the results in a simple grid format.
| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
