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HI-Control 10G Chemically Competent Cells

Product Details

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Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

HI-Control 10G Chemically Competent Cells

Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression.

Key features

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Reduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.
Obtain high protein yields: Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.
Achieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.

Size: 12 reactions (SOLOs)

Item ID 60110-1

TBD

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HI-Control BL21(DE3) Chemically Competent Cells

Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.

Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.

Clone unstable and toxic sequences in standard plasmids, with high efficiency.

Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.

Clone and sub-clone with high-efficiency, recA, endA competent cells.

Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.

Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.

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Product information

The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

	Tightly Control Protein Expression
	T7 Promoters	Non-T7 lac promoters (T5-lac, tac, trc, lac)	Clone with low background target expression	Transformation efficiencies
HI-Control BL21(DE3)	X	X		≥ 1×10⁷ cfu/µg
HI-Control 10G		X	X	≥ 1×10⁹ cfu/µg

Genotypes:

HI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

HI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)

Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.

SDS

Manuals and user guides

HI Control

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Europe, Middle East, and Africa
UK	+44 1992 470 757
Germany	+49 30 5304 2200
North America, Latin America
Wisconsin, USA	+1 888 575 9695
Asia Pacific
China	+8621-22509000
Singapore	+65 6734 4800

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HI-Control 10G Chemically Competent Cells

Product Details

HI-Control 10G Chemically Competent Cells