RapiDxFire qPCR 5X Master Mix + dUTP/UNG (and ROX) is formulated for carry-over amplicon contamination control for use in automated, centralized pathogen testing workflows. This multiplex master mix contains RapiDxFIre Hot Start Taq DNA Polymerase, Uracil-N- Glycosylase (UNG), dNTPs with dUTP, optimized buffer components, and optional ROX reference dye.
RapiDxFire qPCR 5X Master Mix GF has been optimised for use with all Biosearch Technologies’ probes (Dual-Labelled BHQ, BHQplus, BHQnova and BHQplex CoPrimers), which all contain Biosearch Technologies’ BHQ quenchers. BHQ dyes have been proven to dramatically reduce low-level background fluorescence due to highly efficient static quenching between reporter and quencher.
The RapiDxFire qPCR 5X Master Mix, manufactured in an ISO 13485-certified facility, is available in small development sizes to large scale production batches to assist you through the diagnostic test development process.
RapiDxFire qPCR 5X Master Mix with UNG (and ROX) is intended for use as a general purpose reagent in molecular diagnostic assays that are based on qPCR target detection technologies by clinical laboratory professionals located in the United States. This product must be qualified and validated by clinical laboratory end users for suitability in the detection of any specific target using the procedures in this Manual as guidance. This product is classified as a general purpose in vitro diagnostic device reagent in the United States as defined in 21CFR 864.4010.