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  3. Phage Display Library Construction
  4. TG1 Electrocompetent Cells

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TG1 Electrocompetent Cells

TG1 Electrocompetent Cells

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Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

TG1 Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.

Key features

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  • Perfect for antibody phage display library creation
  • The highest efficiency TG1 competent cells available.
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
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SS320 (MC1061 F') Electrocompetent Cells

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SS320 (MC1061 F') Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
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Product information

TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression

Transformation efficiency of Biosearch technologies electrocompetent bacterial cells for phage display compared to competitors

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries


Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

TG1 Electrocompetent

>4 × 1010

YES

NO

YES
IPTG induction required

SS320 (MC1061 F') Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

MC1061 F-Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

ER2738 Electrocompetent

>2 × 1010

YES

NO

YES
IPTG induction required

Genotype

  • TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
  • SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
  • ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1

SDS

Manuals and user guides

Product information sheets

Application notes

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