Phage display is a powerful technique for the selection of peptides, antibodies, or other proteins from a library of millions of candidates. However, its usefulness relies on the ability to generate large and diverse libraries of phage-encoded proteins. Unfortunately, many commercially available competent cells are not well suited for large-scale library construction.
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LGC Biosearch Technologies offers a line of competent cells optimised for large-scale library construction, allowing generation of libraries containing billions of unique phage particles. Designed for high efficiency, you can create the library you need to speed up discovery, opening up a world of possibilities for phage display.
We understand that transformation efficiency is critical for successful experiments. That's why we offer a wide range of competent cells that are rigorously tested to ensure they meet our high standards. Our competent cells must pass strict QC specifications for transformation efficiency and antibiotic resistance. With LGC Biosearch, you can be confident you are using the best possible cells for your project.
| Blue/white capable | Phage T1 resistant | End A-/Rec A | Restriction deficient (RMS) | Clone methylated DNA | Inducible plasmid CN | F’ episome | Antibiotic resistance | Electro-competent efficiency | |
|---|---|---|---|---|---|---|---|---|---|
| TG1 | c | Ø | Ø | d | Ø | Ø | ≥4 × 1010 | ||
| ER2738 | c | c | Ø | Ø | d | Ø | Ø | Tet | ≥2 × 1010 |
| SS320 (MC1061 F’) | c | Ø | Ø | Ø | e | Ø | Ø | Tet, Str | ≥4 × 1010 |
| MC1061 F- | Ø | Ø | Ø | e | Ø | c | Str | ≥4 × 1010 |
“Lucigen’s TGI cells are perfect for phage library construct for their high transformation efficiency.”
– X. W., Molecular Imaging / Department of Radiology
“I have recently tried a sample pack of your Electrocompetent Cells for Phage Display (TG1, ER2738 and SS320). They worked absolutely great! I was quite impressed with the transformation efficiency. No other cells I’ve tested have come close to this!”
– Dr. J. L., Virology, Immunology, and Molecular Microbiology
“The TG1 cells I ordered worked great, and I really appreciate your efforts in getting me the cells in such a short time period. The cells have a super-high transformation efficiency […] and I achieved a much bigger library than I was expecting (great news for me).”
– Wei, Pharmaceutical Researcher
Looking for a custom E. coli strain? We've got you covered! Our team of experts can develop any E. coli strain in flexible formatting options to meet your specific requirements for a wide range of applications.
| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
