The sbeadex Pathogen testing kits have been used successfully with:
|
sbeadex Pathogen Nucleic Acid Purification Kit |
Competitor A |
|
N |
Average Cq |
StDev |
Average Cq |
StDev |
| Clean up sample |
12 |
30.35 |
0.14 |
33.80 |
0.15 |
| Direct spike-in sample |
3 |
31.00 |
0.44 |
33.80 |
0.12 |
Figure 1: Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol. 500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites. The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step
| Pathogen |
External Control (Artificial Matrix) |
| Viral Targets |
|
| SARS-CoV-2 |
VTM1 |
| Influenza A H3N2 |
Swab2 |
| Influenza B |
Swab2 |
| Novel Influenza A H1N1 |
Swab2 |
| Respiratory Syncytial Virus (subtype A) |
Swab2 |
| Respiratory Syncytial Virus (subtype B) |
Swab2 |
| Adenovirus 4 |
Swab2 |
| Zika Virus |
Plasma3 |
| Cytomegalovirus |
Plasma4 |
| Human Papillomavirus-16 |
Methanol-preserved cultured human cells containing full-length HPV episomal DNA5 |
| Bacterial Targets |
|
| Bordetella pertussis |
Swab5 |
| Chlamydophila pneumoniae |
Swab5 |
| Chlamydophila psittaci |
Swab5 |
| Coxiella burnetii |
Swab5 |
| Legionella pneumophila |
Swab5 |
| Mycoplasma pneumoniae |
Swab5 |
| Mycobacterium tuberculosis |
Sputum7 |
| Campylobacter jejuni |
Stool8 |
| Salmonella enteritidis |
Stool8 |
| Yersinia enterocolitica |
Stool8 |
| Clostridium difficile |
Stool8 |
| Chlamydia trachomatis |
Urine9 |
| Neisseria gonorrhoeae |
Urine9 |
| Staphylococcus aureus |
External Run Control10 |
| Other Targets |
|
| Candida albicans |
Positive Extraction Control11 |
| Compatible Matrices Tested |
Swabs (UTM®/VTM) Sputum Saliva Blood Plasma Serum Urine Stool Cerebrospinal Fluid |
Table 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.
Standards used for testing
- AccuPlex™ SARS-CoV-2 Verification Panel - Full Genome, 0505-0168 (LGC SeraCare)
- AMPLIRUN® TOTAL RESPIRATORY VIRAL PANEL CONTROL (SWAB), MBTC020 (Vircell S.L.)
- AMPLIRUN TOTAL ZIKV/DENV/CHIKV CONTROL (PLASMA), MBTC023 (Vircell S.L.)
- AMPLIRUN TOTAL CMV CONTROL (PLASMA), MBTC018-R (Vircell S.L.)
- AccuTrak™ HPV Genotype Qualification Panel, QSH701 (2400-0161), (LGC SeraCare)
- AMPLIRUN TOTAL ATYPICAL BACTERIAL PNEUMONIA CONTROL (SWAB) MBTC022-R (Vircell S.L.)
- AMPLIRUN TOTAL MTB CONTROL (SPUTUM) MBTC013 (Vircell S.L.)
- AMPLIRUN TOTAL GASTROINTESTINAL BACTERIAL PANEL CONTROL (STOOL), MBTC021 (Vircell S.L.)
- AMPLIRUN TOTAL CT/NG CONTROL (URINE), MBTC003 (Vircell S.L.)
- NATtrol™ S. aureus (MRSA) External Run Control, NATSAU(MRSA)-ERCM (Zeptometrix Corporation)
- NATtrol Candida/TV Positive Control, NATCTVPOS-BD (Zeptometrix Corporation)
Note: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.