Education

KASP genotyping

Performing KASP™ genotyping in your own lab is easy. You need your DNA samples, your ordered KASP assays, universal KASP-TF Master Mix from LGC Biosearch Technologies and a FRET-capable plate reader or a qPCR instrument.

Competent cells for larger phage display libraries

Controlled pore glass CPG

End-point PCR with high-throughput instruments

Fluorophores and quenchers

As inventors of the Black Hole Quencher™ (BHQ™) dyes, we design our dyes with the goal of providing solutions for you, the scientist. Our vibrant fluorophores and versatile quenchers are valuable tools for many technologies including: real-time qPCR, multiplex qPCR, SNP genotyping, and FRET assays. See below to learn more about our dyes and the technologies they support.

DNA Synthesis

In addition to being a custom oligonucleotide manufacturer, LGC Biosearch Technologies is a major supplier of DNA synthesis reagents to oligo synthesis companies around the world.

Infographics

Quickly scan and digest topics through an infographic.

Multiplexing qPCR

Multiplexed qPCR combines several PCR assays together into one but resolves the reactions using reporter dyes with distinct spectra. Only Biosearch Technologies offers fluorophores and quenchers that span the spectrum to maximise your multiplexing for 2-plex, 3-plex, 4-plex, and 5-plex qPCR.

Genotyping - qPCR

Real-time PCR methods using fluorescent probes are ideal for end-point genotyping in low to medium throughput disease-association studies, or marker selection in industrial applications. Review the probe types we offer for this application, and learn how to design assays for genotyping.

Gene expression - qPCR

Learn how LGC, Biosearch Technologies can aide your gene expression application.

Quenching mechanisms in oligonucleotide probes

The efficiency of fluorescence quenching upon excitation is very distance dependent – if the reporter fluorophore and quencher are far apart, there is fluorescence; if the reporter and quencher are close together in space, fluorescence is suppressed. Continue reading to learn more about static and FRET quenching.

LGC Biosearch & PCR

The discovery of PCR forever changed the molecular biology world.

Supports and reagents for therapeutic oligonucleotides

Supports and reagents for therapeutic, antisense, and SiRNA oligos. Also dyes and quenchers for bioassays.

Marker-assisted selection (MAS) and Marker-assisted breeding (MAB)

KASP™ genotyping chemistry in MAS allows greater numbers of assays to be rapidly developed and genotyped in a highly flexible and industry proven manner.