FAQ

If you do not have sufficient genomic DNA for the number of SNPs that you wish to run, it is possible to perform Whole Genome Amplification (WGA). WGA is a PCR technique that is used to produce large quantities of DNA from a small amount of starting material. There are a number of methods for WGA, and LGC favours use of the primer extension pre-amplification (PEP) technique. PEP employs the use of randomly synthesised 15-mer oligonucleotides, referred to as polyN15, that bind at sites throughout the genome and act as primers to enable DNA replication. LGC uses our in-house enzyme, Klear Taq, and buffer system to perform WGA reactions.