Contents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.
Number of purifications: 100
DNA and RNA Purification Kits and Reagents
DNA and RNA Purification Kits and Reagents
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2
References
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| Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labelled, and hybridised to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3. | Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Figure. 1. was stored at -20 °C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA. |
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Contact us| Europe, Middle East, and Africa | |
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| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
