Competent Cells include Control DNA and Recovery Medium, and are packaged as FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
We offer two bacterial strains designed for BAC library construction or transformation of large constructs:
BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of CopyRight® Cloning Kits.
E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G BAC-Optimised Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
|
BAC-Optimised Replicator v2.0 Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
Need some support with placing an order, setting up an account, or finding the right protocol?
Contact us| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
