Monkeypox Virus and Orthopoxvirus ValuPanel Reagents are separately delivered probes and primers for PCR detection of monkeypox virus DNA and non-variola Orthopoxvirus species DNA. The oligonucleotide sequences and protocols were designed by the CDC.
Please use the following links for the CDC's PCR protocols:
All primers and probes are HPLC purified and delivered in dried format at 20 nmol of each primer and 5 nmol for each probe. The probes are labelled with FAM dye and BHQ quencher. Probes and primers are delivered in separate tubes and are not premixed.
The Monkeypox Virus and Orthopoxvirus ValuPanel Reagents consist of the following:
Monkeypox virus panel
Oligonucleotides for specific detection of DNA from the monkeypox virus.
| Cat. No. |
Name |
Sequence |
Label |
Nmol delivered |
| MPXV-F-20 |
Monkeypox Virus Forward Primer |
5’-GGA AAA TGT AAA GAC AAC GAA TAC AG-3’ |
None |
20 |
| MPXV-R-20 |
Monkeypox Virus Reverse Primer |
5’-GCT ATC ACA TAA TCT GGA AGC GTA-3’ |
None |
20 |
| MPXV-P-5 |
Monkeypox Virus Probe |
5’-FAM-AAG CCG TAA TCT A(T-BHQ1)GT TGT CTA TCG TGT CC-Spacer C6-3’ |
FAM-BHQ-1 |
5 |
Orthopoxvirus panel (non-variola)
Oligonucleotides for the detection of DNA from Orthopoxviruses excluding smallpox (caused by variola virus).
| Cat. No. |
Name |
Sequence |
Label |
Nmol delivered |
| NVAR-OPXV-F-20 |
Non-Variola Orthopoxvirus Forward Primer |
5’-TCA ACT GAA AAG GCC ATC TAT GA-3’ |
None |
20 |
| NVAR-OPXV-R-20 |
Non-Variola Orthopoxvirus Reverse Primer |
5’-GAG TAT AGA GCA CTA TTT CTA AAT CCC A-3’ |
None |
20 |
| NVAR-OPXV-P-5 |
Non-Variola Orthopoxvirus Probe |
5’-FAM-CCA TGC AAT A (T–BHQ1) A CGT ACA AGA TAG TAG CCA AC-Phos-3’ |
FAM-BHQ-1 |
5 |
Human RNase P extraction control panel
Oligonucleotides for the detection of human RNase P DNA.
| Cat. No. |
Name |
Sequence |
Label |
Nmol delivered |
| RNP-F-20 |
Human RNase P Forward Primer |
5’-AGA TTT GGA CCT GCG AGC G-3’ |
None |
20 |
| RNP-R-20 |
Human RNase P Reverse Primer |
5’-GAG CGG CTG TCT CCA CAA GT-3’ |
None |
20 |
| RNP-P-5 |
Human RNase P Probe |
5’-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1-3’ |
FAM-BHQ-1 |
5 |