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  6. I wish to screen F2 segregating populations in which each SNP can be both homozygous and heterozygous. Will Flex-Seq provide enough depth of coverage to allow for this level of discrimination?

FAQ

I wish to screen F2 segregating populations in which each SNP can be both homozygous and heterozygous. Will Flex-Seq provide enough depth of coverage to allow for this level of discrimination?

For Flex-Seq the standard targeted depth per locus is a range between 75-100x. This depth is the average for the entire panel, which means some loci will receive higher or lower depth. Accurate SNP calling is routinely possible with 10x coverage per haploid copy of the genome.