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  6. How much DNA do I need to run on an agarose gel to initially assess whether my DNA is suitable for Flex-Seq?

FAQ

How much DNA do I need to run on an agarose gel to initially assess whether my DNA is suitable for Flex-Seq?

We recommend running 5 – 10 ng per sample of DNA using a high range marker to visualise the integrity of the DNA. To check quantity we strongly recommend using an intercalating dye/fluorometric quantification and actively discourage use of spectrophotometric methods. DNA which appears to ‘smear’ down the gel is indicative of degraded genomic DNA, whereas DNA which generates a tight band is indicative of intact DNA. 

We recommend DNA be extracted using methods that yield higher quality DNA, such as column, bead based, or C-TAB extraction. We would discourage use of "crude" DNA extractions.