1. Home
  2. Support
  3. FAQs
  4. Flex-Seq FAQs
  5. Assay design
  6. I have determined how many markers I wish to characterise using Flex-Seq. What is the recommended number of candidates to ensure successful probe design across all my markers?

FAQ

I have determined how many markers I wish to characterise using Flex-Seq. What is the recommended number of candidates to ensure successful probe design across all my markers?

It is advisable to send at least 2x – 3x the number of candidates for the number of markers you wish you interrogate (e.g. for 1000 markers, please submit 2000 – 3000 candidates). This will increase the chances that a greater number of probes (i.e. >1000) will be generated in the first design iteration. Please note, design success is variable based on the candidates chosen and the genome of the organism.