FAQ

All SNPs identified for interrogation by Flex-Seq must achieve a minimum of 10x coverage (a minimum of 3 reads per allele, for a bi-allelic SNP), otherwise the target SNP is not assigned a genotype. If greater confidence is desired, an increased minimum coverage can be applied and this must be discussed prior to the commencement of the project. We will recommend that an appropriate level of multiplexing is performed in relation to the capacity of the sequencing run to ensure the minimum coverage per sample is met.