KASP-TF (Triton-Free) Master Mixes
What you need to know about the new KASP-TF Master Mixes and how we extensively validated performance
Topics covered
- Why we updated KASP Master Mix formulations and what changes to expect
- How KASP-TF formulations meet and exceed our rigorous testing standards
- Data from our comprehensive validation experiments
Why were KASP-TF Master Mixes developed?
KASP Master Mix formulations have been updated to remove Triton X-100 in compliance with the REACH Regulation for Registration, Evaluation, Authorization and Restriction of Chemicals ((EC) No 1907/2016), which was implemented by the European Commission to protect human health and the environment through the safe use of chemicals. As of January 04, 2021, use of nonylphenol ethoxylate (NPE) and octylphenol ethoxylate (OPE) based surfactants such as Triton-X100 are not permitted in the EU, unless an authorization is granted or the use is exempted from authorization. The updated KASP-TF Master Mixes are free of Triton-X100, with the same performance and quality you have always expected from your KASP genotyping mix.
Collect data for any template sequence or scale
The reformulated KASP™-TF Master Mix enables you to obtain reliable clustering and collect high-quality data you know and trust — no matter the template or scale.
We performed extensive validation testing to cover the wide range of sample types our customers study to confirm that the new formulation does not have any impact on the end-point genotyping data in comparison to the old KASP Master Mix formula.
Download application noteKASP-TF Master Mix validation in numbers
Tissue types: 6
Extraction methods: 4
Sample sets (tissue type + extraction method): 10
Genotyping platforms: 3
Unique samples: 986
Plates and arrays run: 862
Total number of data points: 585,600
Run your assay, your way
No matter what the scale, platform, DNA extraction method, species, or template sequence, our experienced scientific support empowers you to successfully develop and troubleshoot your KASP process.
In order to ensure your genotyping workflows remain uninterrupted, we are equipping you with our recommendations for setting up your comparative validation experiments.
Download quick guideConsult our experienced scientific support team
We act as your support system, not just for the technology but also for your applications.
Please contact us with your questions.
Additional resources
KASP-TF Master Mix
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Download slides
KASP Master Mix Triton-free formulations: complying with new regulations
Read the App NoteQuick guide for validating KASP Triton-free Master Mix
Download quick guideKASP-TF Master Mix Catalog Numbers
Find your recommended KASP-TF Master Mix in the table below. You can also refer to the KASP genotyping protocols for your PCR instrument to select the correct KASP-TF Master Mix for your instrument.
| Discontinued KASP Master Mixes | New KASP-TF Master Mixes | ||
|---|---|---|---|
| Catalog Number | Description | Catalog number | Description |
| KBS-1016-001 | KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL | KBS-1050-101 | KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (2.5 mL) |
| KBS-1016-001-US | KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU | ||
| KBS-1016-002 | KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL | KBS-1050-102 | KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (25 mL) |
| KBS-1016-002-US | KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU | ||
| KBS-1016-003 | KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL | KBS-1050-103 | KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL) (10 x 25 mL) |
| KBS-1016-003-US | KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU | ||
| KBS-1016-003B-US | KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU | KBS-1050-104 | KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL) |
| KBS-1016-010 | KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL | KBS-1050-111 | KASP-TF V4.0 2X Master Mix 1536, Standard ROX (2.5 mL) |
| KBS-1016-010-US | KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL USA/AU | ||
| KBS-1016-011 | KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL | KBS-1050-112 | KASP-TF V4.0 2X Master Mix 1536, Standard ROX (25 mL) |
| KBS-1016-011-US | KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL USA/AU | ||
| KBS-1016-012 | KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 10 x 25 mL | KBS-1050-113 | KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL) (10 x 25 mL) |
| KBS-1016-012-US | KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 250 mL - 10 x 25 mL USA/AU | ||
| KBS-1016-012B | KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL | KBS-1050-115 | KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL) |
| KBS-1016-012B-US | KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL USA/AU | ||
| KBS-1016-016 | KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL | KBS-1050-121 | KASP-TF V4.0 2X Master Mix 96/384, Low ROX (2.5 mL) |
| KBS-1016-016-US | KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU | ||
| KBS-1016-017 | KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL | KBS-1050-122 | KASP-TF V4.0 2X Master Mix 96/384, Low ROX (25 mL) |
| KBS-1016-017-US | KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU | ||
| KBS-1016-018 | KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL | KBS-1050-123 | KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL) (10 x 25 mL) |
| KBS-1016-018-US | KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU | ||
| KBS-1016-018B-US | KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU | KBS-1050-124 | KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL) |
| KBS-1016-021 | KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL | KBS-1050-131 | KASP-TF V4.0 2X Master Mix 96/384, High ROX (2.5 mL) |
| KBS-1016-021-US | KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU | ||
| KBS-1016-022 | KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL | KBS-1050-132 | KASP-TF V4.0 2X Master Mix 96/384, High ROX (25 mL) |
| KBS-1016-022-US | KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU | ||
| KBS-1016-023 | KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL | KBS-1050-133 | KASP-TF V4.0 2X Master Mix 96/384, High ROX (250 mL) (10 x 25 mL) |
| KBS-1016-023-US | KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL USA/AU | ||
| KBS-1030-001 | KASP Array Tape 2X Master Mix, Standard ROX, - 2.5 mL | KBS-1050-201 | KASP-TF V5.0 2X Master Mix, Standard ROX (2.5 mL) |
| KBS-1030-002 | KASP Array Tape 2X Master Mix, Standard ROX, - 25 mL | KBS-1050-202 | KASP-TF V5.0 2X Master Mix, Standard ROX (25 mL) |
| KBS-1030-004 | KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL (10 x 25 mL) | KBS-1050-203 | KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL) (10 x 25 mL) |
| KBS-1030-003 | KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL | KBS-1050-204 | KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL) |