RNA FISH for mouse
About 25% of publications citing Stellaris™ RNA FISH use mouse targets, making this the second most common target after humans.
Frozen mouse brain
Olig2 mRNA
A huge variety of mouse cell types and tissues have been used with Stellaris RNA FISH probe sets and published successfully by our customers including the brain, embryo, retina, lungs, liver, placenta, ovary, eggs and sperm, intestines, and nerves. Stellaris has also been demonstrated to be compatible with the tissue clearing technology CLARITY, making it possible to probe and image RNA transcripts in an entire intact organ. If none of the professionally-designed sets meet your needs, you can always custom design one using our online designer.
Specific mouse resources
Positive control probe sets
We have positive control ShipReady probe sets for mouse targets ranging from a qualitative Gapdh controls to quantitative spot counting controls like Trfc. ShipReady assays are offered at a fraction of the price of custom or DesignReady probe sets, target constitutively active genes, and should work with almost all mouse samples. We verify these probe sets on cells and/or tissues in house and provide images on the product information sheet of their staining patterns. To view the complete control collection and use our interactive selection guide, click below.
Professionally-designed mouse-specific probe sets include
Mouse brain
Plp1 mRNA labeled with Stellaris RNA FISH probes (green), DAPI (red) and imaged with Stellarvision
For more information on the Stellarvision imaging platform, email Optical Biosystems.
Mouse embryonic stem cells
Nanog (red), Oct4 (green), Sox2 (yellow)
Courtesy of: Ana Guedes
Mouse-specific support materials
Protocols
Image gallery: Customer Submitted Images of Stellaris RNA FISH in mouse cells and tissues
Technical Support: If required, contact techsupport@biosearchtech.com for assistance with this application.
Publication highlights
Highlighted neuroscience publications citing Stellaris RNA FISH probe sets.
Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science
Image of simultaneous RNA FISH and immunofluorescence assay. eGFP RNA molecules (red), protein marker GFP (green), DAPI (blue) in mouse E13 primary DRG neuron culture cells (submitted by Adi Minis).
Visit our Citation Center for the complete list.
CLARITY Publication: Multiplexed Intact-Tissue Transcriptional Analysis at Cellular Resolution. Emily Sylwestrak et al. Cell. 2016.
Super-resolution imaging of fluorescently labelled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing. Cho et al. Nature Scientific Reports. 2016.
Wnt Ligands Secreted by Subepithelial Mesenchymal Cells Are Essential for the Survival of Intestinal Stem Cells and Gut Homeostasis. Valenta et al. Cell Reports. 2016.
An alternative pluripotent state confers interspecies chimaeric competency.. Wu et al. Nature. 2015.