The I-Q570 modification incorporates a fluorescent Quasar® 570 dye internally or at the 5’ terminus of an oligonucleotide. Quasar 570 modified oligonucleotides can be used in a wide array of applications including dual-labeled fluorogenic probes for real-time PCR. This fluorophores is conjugated to a modified thymidine which may participate in hybridization. Quasar 570 fluorophore is an indocarbocyanine dye which fluoresces in the yellow-orange region of the visible spectrum and can be effectively quenched by BHQ®-2 dye. It is used as a direct replacement for Cy™ 3 dye.
Properties:
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Chemical Name:
T(Quasar® 570)
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Formula:
C49H63N6O10P
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Molecular Weight:
927.03
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Absorption Maximum (Lambda Max):
548
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Extinction Coefficient at Lambda max:
118000
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Extinction Coefficient at 260 nm:
20000
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Fluorescence Maximum:
566
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
912.03
