The 3-CO modification adds a fluorescent CAL Fluor® Orange 560 dye to the 3’ end of an oligonucleotide. CAL Fluor Orange 560 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. The fluorophore is conjugated to a modified thymidine which may participate in hybridization. CAL Fluor Orange 560 fluorophore is a xanthene dye that fluoresces in the orange region of the visible spectrum and can be effectively quenched by BHQ®-1 dye. It is used as a replacement for the VIC, HEX and JOE dyes.
Properties:
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Chemical Name:
T(CAL Fluor® Orange 560)
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Formula:
C51H64N7O14P
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Molecular Weight:
1030.07
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Absorption Maximum (Lambda Max):
538
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Extinction Coefficient at Lambda max:
87600
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Extinction Coefficient at 260 nm:
28500
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Fluorescence Maximum:
559
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1094.05
