The 5-CG modification adds a fluorescent CAL Fluor® Gold 540 dye to the 5’ end of an oligonucleotide. CAL Fluor Gold 540 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. CAL Fluor Gold 540 fluorophore is a xanthene dye that fluoresces in the yellow region of the visible spectrum and can be effectively quenched by BHQ®-1dye. It is used as a replacement for the TET dye.
Properties:
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Chemical Name:
CAL Fluor® Gold 540
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Formula:
C29H29N2O7P
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Molecular Weight:
548.52
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Absorption Maximum (Lambda Max):
522
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Extinction Coefficient at Lambda max:
80100
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Extinction Coefficient at 260 nm:
15100
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Fluorescence Maximum:
544
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
532.52
