Skip to content Skip to navigation menu
New Site Launched: Looking for oligos? Visit our new oligo and Stellaris dedicated platform at oligos.biosearchtech.com
 
cancel
  1. Home
  2. sbeadex Blood FAQs

sbeadex Blood FAQs

sbeadex blood has been tested with whole blood (fresh and frozen), buffy coats and FTA cards. It has been used successfully with common anticoagulants: EDTA, Heparin, and Citrate.

Sample type

Validated

Notes

Fresh & frozen blood

100 & 200 µl

All other volumes: Please contact us for additional information

Buffy coats

Buffy coats of 200 µl starting volume

Buffy coats can be very viscous due to the concentration of white blood cells, so may need dilution. See the protocol for details.

FTA cards

Typically: 3 mm punch
  • Protocol can be upscaled to include several punches
  • FTA cards can store varying amounts of blood depending on the technology used, so some testing may be needed.

Plasma & serum

Pending validation

We have not fully validated plasma and serum samples; please call us to discuss.

sbeadex blood is compatible with most liquid handling platforms. At this time, protocols are available for the Kingfisher 96. Please contact us for information about other systems.

sbeadex blood provides high quality DNA suitable for all downstream applications, including NGS. If your current extraction technology is failing because of sample quality, sbeadex blood will resolve the issue.

However, additional causes of failure maybe:

Old/degraded blood samples
  • DNA fragments may be too degraded or too small to function in downstream application.
  • Blood clotting might be a problem.
  • Test by performing control experiments with fresh blood or spiked samples.

Storage medium and method used to store blood samples
  • sbeadex blood is compatible with all common anticoagulants such as EDTA, Heparin, Citrate.
  • sbeadex blood is compatible with whole blood: fresh and frozen, buffy coats, FTA cards, Plasma/ Serum.
  • Typical storage conditions:
    • Approx. 1-4 weeks at 4°C
    • Approx. 1-12 months at -20°C
  • Longer storage may impact DNA quality.

Method (not followed)/ requires adaptation
  • Please describe your sample matrices (volume; storage, etc) and results required, also review method used.
  • Be sure to follow the protocol provided with Sbeadex blood kit unless we give specific advice to optimise for your needs.

Downstream assay not working/ highly sensitive to input DNA quality
  • Ethanol carryover in final step can cause reaction failure. sbeadex blood does not use ethanol in final step before elution, making this problem less likely.
  • Use controls with downstream assay to confirm assay performance It is also possible to use controls in your extraction process.

Low DNA concentrate in downstream assays
  • In our validation studies, sbeadex blood provides 4 to 12 µg, average yield 6.7 µg across sample types listed using the standard protocol. Providing there is adequate accessible DNA in the sample extracted, sbeadex blood should provide adequate DNA for most downstream applications.

sbeadex blood gives high levels of purified ds DNA with an approx. size around 30-50kB.

DNA extracted with sbeadex has been used to construct NGS libraries. The DNA is highly purified, due to:

  • A 2-step binding mechanism for removing impurities
  • No use of organic solvents or chaotrophic salts in the final step before elution

It is therefore highly suitable for NGS.

Yields will depend on quality of blood sample and also storage conditions and time stored. As a rough guide, see the table below.

However, we recommend you try sbeadex blood to get a more accurate idea of yields in your laboratory with your sample type.

Sample

DNA yields

Notes

200 µl fresh whole blood

4 to 12 µg, average yield 6.7 µg

200 µl frozen whole blood

3-8 µg

Yields lowered because white blood cells are destroyed by freezing / thawing the sample; DNA gets lost

20 µl buffy coat (from 200 µl blood)

4-10 µg

Slight loss of DNA in buffy coat preparation

1 FTA punch

100 ng +

Difficult to estimate as various FTA cards have differing collection capacities. Please discuss your needs with us.

200 µl plasma/ serum

No data

Yes, we frequently work with customers to optimise kits when there are unique requirements. Depending on what is required, there may be a charge for this.

Yes, it is possible to organise custom configurations and dispensing, depending on purchase volume. Contact us to discuss your needs.

The kits have been validated for up to 1 year’s stability when stored at -20C.

Common blood collection vessels with anticoagulants EDTA, Citrate and Heparin are compatible with sbeadex blood.

For manual processes you will need:

  • Plates (1.2 ml well volume or Eppendorf (1.5 mls)
  • Magnetic block – if this is not possible then you can use a centrifuge

If you wish to automate sbeadex blood, please get in touch to discuss your platform needs.

Product

Cat. No.

Size (rxn)

sbeadex blood kit

NAP44401

96

NAP44410

960

NAP44100

5 000

sbeadex blood kit APAC

NAP44404

96

NAP44440

960

NAP44400

5 000

APAC kits do not contain any alcohols and do not need to be shipped as dangerous goods.