RNA FISH in plants
Visualization and quantification of single RNA transcripts in plants, using Stellaris™ RNA fluorescence in situ hybridisation (RNA FISH) using smFISH technology, is an exciting new application. Publications and support materials are now available to assist with this application.
Custom Stellaris Probe sets can be designed against almost any RNA target using our free online designer. As we do not yet have plant genomes on the online designer, we recommend following the steps in this technical blog to optimise your probe set and help you decide which probe sequences to choose. The Stellaris buffer set streamlines your experiments and improves the quality of your images by decreasing troublesome background fluorescence.
Arabidopsis thaliana root meristem tissue
CYCA3;1 mRNA (red), CDKB2;1 mRNA (green)
Courtesy of: Congyan Xu
Learn about the Stellaris RNA FISH technology in this entertaining yet informative video that contains 3 segments: introduction, animated RNA FISH mechanism, and interview portion discussing the applications of Stellaris FISH.
Plant-specific support materials:
A protocol for the use of Stellaris RNA FISH for detection of single molecules of mRNA in Arabidopsis thaliana root meristem was generously submitted by expert user Susan Duncan (see publications below).
Protocol: Protocol for Arabidopsis thaliana root meristem
Technical Support: Contact techsupport@biosearchtech.com for assistance with plant-based applications.
Image Gallery: Customer Submitted Images of Stellaris RNA FISH in plant cells and tissues
Arabidopsis thaliana root meristem tissue
FLC mRNA (green)
Courtesy of: Stefanie Rosa
Comparison of traditional and LGC Biosearch buffers in Arabidopsis tissue
Arabidopsis thaliana root meristem tissue - PP2A mRNA (red)
Courtesy of: Stefanie Rosa who both performed this comparison and shared these images.
A) Homemade buffer mix used with Stellaris RNA FISH probes (left).
B) Stellaris buffer set used with Stellaris RNA FISH probes (right).
Publication Highlights
Highlighted plant publications citing Stellaris RNA FISH probe sets. Over 33% of publications citing Stellaris RNA FISH publish in Cell, Nature, or Science
Visit our Citation Center for the complete list.
Arabidopsis thaliana root meristem tissue
PP2A mRNA (red), FLC mRNA (green)
Courtesy of: Stefanie Rosa and Susan Duncan
Cell-Size-Dependent Transcription of FLC and Its Antisense Long Non-coding RNA COOLAIR Explain Cell-to-Cell Expression Variation. Robert Ietswaart et al. Cell Systems. 2017.
Single Molecule RNA FISH in Arabidopsis Root Cells. Susan Duncan et al. Bio-Protocol. 2017.
Mutually exclusive sense-antisense transcription at FLC facilitates environmentally induced gene repression. Stefanie Rosa et al. Nature Communications. 2016.
A method for detecting single mRNA molecules in Arabidopsis thaliana. Claudia Susan Duncan et al. Plant Methods. 2016.
Lignin and lignans in plant defence: Insight from expression profiling of cinnamyl alcohol dehydrogenase genes during development and following fungal infection in Populus. Agnieszka Bagniewska-Zadworna et al. Plant Science. 2014.