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Stellaris® FISH Probes, Human ACTB with Quasar® 670 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect ACTB transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against actin, beta ACTB a.k.a. BRWS1; PS1TP5BP1 (NCBI gene ID:60) and nucleotides 337-1811 of NM_001101.3 . This is an inclusive probe set designed to also detect the following variants: NM_001101.3, XM_006715764.1, AK309997. Due to sequence similarities, potential cross-hybridization to RNAs from the following genes may occur: ACTA1, ACTA2, ACTB, ACTBL2, ACTC1, ACTG1, ACTG2, ACTL6A, ACTL6B, ACTL7A, ACTL7B, ACTL8, ACTL9, ACTL10, ACTR5, ACTR8, ACTRT2, POTEA, POTEB, LOC102724631, POTEB2, POTEC, POTED, LOC100288966, POTEE, POTEF, POTEG, POTEH, POTEI, POTEJ, POTEM, LOC100508046. Note that due to potential cross-hybridization to RNA from other genes, this probe set is best used as a positive control for establishing the Stellaris RNA FISH method and not for single molecule quantification. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
647
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Fluorescence Maximum:
670
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
