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Monkeypox Virus and Orthopoxvirus ValuPanel Reagents

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Monkeypox Virus and Orthopoxvirus ValuPanel™ Reagents are separately delivered probes and primers for PCR detection of the monkeypox virus and non-variola Orthopoxvirus species.

The disease mpox (formerly known as monkeypox) is caused by the monkeypox virus, a double-stranded DNA virus that belongs to the Orthopoxvirus genus in the family Poxviridae. The Orthopoxvirus genus also includes the cowpox, variola (which causes smallpox) and vaccinia viruses (used in the smallpox vaccine). Mpox is endemic in central and western Africa, but in 2022-2023 a global outbreak marked the first widespread transmission outside of Africa. 

For Research Use and Surveillance Only. Not for use in diagnostic procedures.

Key benefits

  • Reliable results: From the original manufacturer of Black Hole Quencher™ (BHQ™) technology.
  • Consistent quality: All probes and primers are HPLC purified and are manufactured and shipped from a facility entirely separate from positive control production.
  • Flexible assay design: Increased assay design options with separately delivered probes and primers. 
  • Fast delivery: Product in stock ships the next business day.

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Product details

Monkeypox Virus and Orthopoxvirus ValuPanel Reagents are separately delivered probes and primers for PCR detection of monkeypox virus DNA and non-variola Orthopoxvirus species DNA. The oligonucleotide sequences and protocols were designed by the CDC.

Please use the following links for the CDC's PCR protocols:

All primers and probes are HPLC purified and delivered in dried format at 20 nmol of each primer and 5 nmol for each probe. The probes are labelled with FAM dye and BHQ quencher. Probes and primers are delivered in separate tubes and are not premixed.

The Monkeypox Virus and Orthopoxvirus ValuPanel Reagents consist of the following:

Monkeypox virus panel

Oligonucleotides for specific detection of DNA from the monkeypox virus.

Cat. No. Name Sequence Label Nmol delivered
MPXV-F-20 Monkeypox Virus Forward Primer 5’-GGA AAA TGT AAA GAC AAC GAA TAC AG-3’ None 20
MPXV-R-20 Monkeypox Virus Reverse Primer 5’-GCT ATC ACA TAA TCT GGA AGC GTA-3’ None 20
MPXV-P-5 Monkeypox Virus Probe 5’-FAM-AAG CCG TAA TCT A(T-BHQ1)GT TGT CTA TCG TGT CC-Spacer C6-3’ FAM-BHQ-1 5

 

Orthopoxvirus panel (non-variola)

Oligonucleotides for the detection of DNA from Orthopoxviruses excluding smallpox (caused by variola virus).

Cat. No. Name Sequence Label Nmol delivered
NVAR-OPXV-F-20 Non-Variola Orthopoxvirus Forward Primer 5’-TCA ACT GAA AAG GCC ATC TAT GA-3’ None 20
NVAR-OPXV-R-20 Non-Variola Orthopoxvirus Reverse Primer 5’-GAG TAT AGA GCA CTA TTT CTA AAT CCC A-3’ None 20
NVAR-OPXV-P-5 Non-Variola Orthopoxvirus Probe 5’-FAM-CCA TGC AAT A (T–BHQ1) A CGT ACA AGA TAG TAG CCA AC-Phos-3’ FAM-BHQ-1 5

 

Human RNase P extraction control panel

Oligonucleotides for the detection of human RNase P DNA.

Cat. No. Name Sequence Label Nmol delivered
RNP-F-20 Human RNase P Forward Primer 5’-AGA TTT GGA CCT GCG AGC G-3’ None 20
RNP-R-20 Human RNase P Reverse Primer 5’-GAG CGG CTG TCT CCA CAA GT-3’ None 20
RNP-P-5 Human RNase P Probe 5’-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1-3’ FAM-BHQ-1 5

Resources

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Note: Optimization may be required for related products as ValuPanel oligonucleotides are agnostic to specific reagents and workflows.

*For Research Use Only. Not for use in diagnostic procedures.