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Stellaris® FISH Probes, Human GLI3 with FAM consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect GLI3 transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against GLI family zinc finger 3 GLI3 a.k.a. PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190 (NCBI gene ID:2737) and nucleotides 218-4834 of NM_000168.5. This is an inclusive probe set design to detect the following variants: NM_000168.5, XM_005249703.1, XM_005249704.2, XM_011515272.1, XM_011515273.1, XM_011515274.1. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
495
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Fluorescence Maximum:
520
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
