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The 5-ATTO 532 modification incorporates an ATTO™ 532 dye (ATTO-TEC GmbH) at the 5' terminus of an oligonucleotide. With excellent water solubility, strong absorption and high fluorescence quantum yield, ATTO 532 can be used in a wide array of applications, including single molecule detection, high-resolution microscopy, flow cytometry (FACS), and fluorescence in-situ hybridization (FISH). ATTO 532 can be used as a substitute for VIC®; particularly where enhanced photostability is desired. ATTO 532 fluoresces in the green region of the visible spectrum and its fluorescence can be effectively quenched by BHQ®-1 dye.

Properties:

  • Formula:

    C35H44N4O13PS2

  • Molecular Weight:

    823.85

  • Absorption Maximum (Lambda Max):

    532

  • Extinction Coefficient at Lambda max:

    115000

  • Extinction Coefficient at 260 nm:

    25300

  • Fluorescence Maximum:

    553

Product usage:

  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

    807.85

Storage and handling: