The 3-ATTO 647N mdCNH modification incorporates an ATTO™ 647N dye (ATTO-TEC GmbH) at the 3' terminus of an oligonucleotide. ATTO 647N modifications exhibit high thermo, pH, and photo-stability. With strong absorption and high fluorescence quantum yield, ATTO 647N can be used in a wide array of applications, including single molecule detection, high-resolution microscopy, and fluorescence in-situ hybridization (FISH). This 3' ATTO 647N modification is conjugated to a modified cytidine which may participate in hybridization. ATTO 647N is a cationic dye and can be used as a substitute for Alexa Fluor® 647, Cy5™, and Quasar® 670; particularly where enhanced photostability is desired. Fluorescing in the red region of the visible spectrum, ATTO 647N can be effectively quenched by BHQ®-2 dye.
Properties:
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Formula:
C62H85N7O9
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Molecular Weight:
1072.38
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Absorption Maximum (Lambda Max):
644
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Extinction Coefficient at Lambda max:
150000
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Extinction Coefficient at 260 nm:
9000
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Fluorescence Maximum:
669
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1135.35
