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5'-DMT-dA(Bz) "Twist" Column; 1000 Å is a 10 µmol column packed with 5'-DMT-dA(Bz)-Suc-CPG, which is used for the placement of a deoxyadenosine (dA) base on the 3' end of the oligonucleotide sequence. The 5'-DMT-dA(Bz)-Suc-CPG support is linked to the CPG support through a succinyl linkage which can be cleaved in concentrated NH4OH or Methyl amine/ NH4OH(AMA). The dA has a benzoyl protecting group which is removed during the deprotection step using concentrated NH4OH or AMA. This support is compatible with fast deprotecting amidites and chemistry.
Properties:
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Appearance:
white powder
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Extinction Coefficient at 260 nm:
15200
Spectral properties measured in water of cleaved and deprotected nucleoside.
Product usage:
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Synthesis conditions:
Follow the instrument manufacturer's protocol, with noted changes, when using this product. When setting up the instrument for synthesis choose the 10µmol or 15µmol program for a successful synthesis using these columns
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Cleavage conditions:
Use concentrated ammonia for 30 minutes at 65 °C or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C.
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Deprotection conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
233.24
Image of cleaved and deprotected structure:
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+5 °C in sealed bag or container
