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Properties:
-
Appearance:
dark red powder
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Absorption Maximum (Lambda Max):
534
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Extinction Coefficient at Lambda max:
34000
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Extinction Coefficient at 260 nm:
8000
Spectral properties measured in PCR buffer as 3'-labeled poly(T) oligo.
Product usage:
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Cleavage conditions:
When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
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Deprotection conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 12-15 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
554.49
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
-15 to -30 °C in sealed container
