The 3-BHQ2 modification adds a non-fluorescent Black Hole Quencher®-2 (BHQ®-2) dye to the 3’ end of an oligonucleotide. 3' BHQ-2 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. The BHQ-2 dark quencher has strong absorption from 599 nm to 670 nm, which provides excellent quenching of fluorophores that fluoresce in this range, such as Quasar® 570, TAMRA, CAL Fluor® Red 590, CAL Fluor Red 610, ROX, CAL Fluor Red 635, Pulsar® 650, Quasar 670 and Quasar 705 dyes. BHQ-2 dye may quench fluorescence by both FRET and static quenching mechanisms.
Properties:
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Chemical Name:
BHQ®-2
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Formula:
C24H25N6O6
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Molecular Weight:
493.49
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Absorption Maximum (Lambda Max):
579
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Extinction Coefficient at Lambda max:
38000
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Extinction Coefficient at 260 nm:
8000
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
556.46
