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5'-DMT-dG(DMF)-Suc-CPG is used for the placement of dG on the 3' end of oligonucleotides. The 1000 Å CPG is best suited for the synthesis of DNA sequences up to 100-mers in length.
Properties:
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Appearance:
White Powder
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Extinction Coefficient at 260 nm:
12010
Product usage:
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Cleavage conditions:
Use concentrated ammonia for 90 minutes at 25° C or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25° C.
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Deprotection conditions:
When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60° C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60° C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
329.2
Image of cleaved and deprotected structure:
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
