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5'-DMT-dC(Bz)-Suc-CPG is used for the placement of dC on the 3' end of oligonucleotides. The 1000 Å is best suited for the synthesis of DNA sequences up to 100-mers in length.
Properties:
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Chemical Name:
5'-DMT-dC(Bz)-Suc-CPG; 1000 Å
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Appearance:
white powder
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Extinction Coefficient at 260 nm:
7050
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product usage:
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Cleavage conditions:
Cleave in concentrated ammonia for 90 minutes at 25°C.
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Deprotection conditions:
Deprotect in concentrated ammonia for 5 hours at 60 °C.
Use only concentrated NH4OH only with this product.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
289.18
Image of cleaved and deprotected structure:
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
