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TFA-Aminohexyl Amidite can be used to produce a functional amine group on the 5' end of an oligonucleotide. The base-labile TFA protecting group is cleaved in solution using normal ammonia deprotection and cleavage conditions.
Properties:
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Chemical Name:
(N-trifluoroacetyl-6-aminohexyl)-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
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Formula:
C17H31F3N3O3P
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Molecular Weight:
413.42
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Appearance:
colorless oil
Product usage:
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Synthesis conditions:
Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.
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Dilution:
100 µmol/mL
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Deprotection conditions:
The deprotecting conditions for this amidite will be depend on the types of amidites used for the synthesis of the oligo. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60°C. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60°C. The TFA group will be eliminated during ammonia deprotection.
The base labile protecting group comes off during ammonia deprotection. The crude functionalized 5’ amine oligo may be reacted with the labeling compound and then purified using RP Cartridge purification.
Image of cleaved and deprotected structure:
Storage and handling:
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Storage conditions:
-20 °C, desiccated
