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3'-DMT-dG(iBu)-Suc-CPG; 1000 Å is an inverse linkage CPG used to create a 3'-3' linkage on the end of an oligonucleotide. An inverted 3'-3' linkage is desirable when needing to block polymerase extension through the 3'-terminus of a PCR probe; additionally, oligonucleotides with inverted 3'-linkages are used for some sequencing techniques. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length.
Properties:
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Chemical Name:
3'-DMT-dG(iBu)-Suc-CPG; 1000 Å
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Appearance:
White Powder
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Extinction Coefficient at 260 nm:
12010
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product usage:
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Protecting group deblock conditions:
3'DMT groups detritylate slowly. Increase detritylation time by 50%.
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Cleavage conditions:
Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C.
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Deprotection conditions:
Use concentrated ammonia for 5 hours at 60 °C.
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
329.2
Image of cleaved and deprotected structure:
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
