FAQ

The static quenching mechanism is the formation of an intramolecular dimer between reporter and quencher, to create a non-fluorescent ground-state complex with a unique absorption spectrum. In contrast, the FRET quenching mechanism is dynamic and does not affect the probe's absorption spectrum. With either mechanism, disruption of quenching through hydrolysis of the probe releases signal from the fluorophore.

For more information, please visit our Quenching Mechanisms in Probes webpage.